|
KMID : 0982820060050020111
|
|
Journal of Lung Cancer
2006 Volume.5 No. 2 p.111 ~ p.113
|
|
|
Pro-apoptotic Cytochrome c Gene Mutation is Rare in Non-small Cell Lung Cancers
|
|
Soung Young-Hwa
Lee Sug-Hyung
|
|
|
Abstract
|
|
|
Purpose: Several lines of evidence have indicated that the deregulation of apoptosis is involved in the mechanisms of cancer development, and somatic mutations of the apoptosis-related genes have been reported in human cancers. In addition to its role in oxidative phosphorylation, release of cytochrome c from the mitochondrial intermembrane space results in nuclear apoptosis. The aim of this study was to explore whether alteration of cytochrome c gene mutation is a characteristic of human non-small cell lung cancers (NSCLC).
Materials and Methods: In the current study, to detect the somatic mutations in the DNA sequences encoding cytochrome c in 48 NSCLCs, we used polymerase chain reaction (PCR), single-strand conformation polymorphism (SSCP), and DNA sequencing.
Results: The SSCP analysis revealed no mutation in the entire coding regions and all splice sites of human cytochrome c gene in the 48 NSCLCs.
Conclusion: The data presented here indicate that the pro-apoptotic cytochrome c may not be somatically mutated in human NSCLCs, and suggest that NSCLCs may not utilize mutational events of cytochrome c gene in the mechanisms for evading apoptosis.
|
|
|
KEYWORD
|
|
|
Non-small cell lung cancer, Cytochrome c, Apoptosis, Mutation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|